Calibration will be prompted at every device/app connection, and can be manually triggered from the Manage Device screen in the app. Calibrate prior to each scan for the highest degree of accuracy. If you are using your device for many scans in a row, it is recommended that you calibrate it after every ten to twenty scans. After calibration is complete, remove the end cap, place it on the back of your device, and you're ready to scan.
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